HTK solution helps to preserve endothelial integrity of saphenous vein: an immunohistochemical and ultrastructural analysis.
J Biol Regul Homeost Agents
; 25(1): 93-9, 2011.
Article
en En
| MEDLINE
| ID: mdl-21382278
ABSTRACT
The aim of the study is to demonstrate the ability of HTK (Histidine-tryptophan-ketoglutarate) solution to preserve endothelium. Ten saphenous veins (SVs) were prospectively collected from 10 patients who underwent coronary artery bypass grafting (CABG). The SVs were divided into two sets of segments, one of which preserved in HTK solution at 4°C (group A), and the other preserved at 4°C in saline solution NaCl 0.9% (group B); ten pieces from the SVs were processed as control. The control sample was fixed in 10% neutral buffered formalin immediately after harvesting. The observation lasted up to the 5th postoperative day. A morphological, ultrastructural, and immunohistochemical analysis (CD31) was performed on each piece. Immunohistochemical analysis demonstrated significant protection on endothelium in group A compared to group B starting from the 1st observational day. Ultrastructural data confirmed immunohistochemistry. These preliminary results represent a basis for further analysis. They suggest the protective role of HTK solution in preserving endothelial integrity and may imply some clinical benefits in organ protection.
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Base de datos:
MEDLINE
Asunto principal:
Preservación de Órganos
/
Vena Safena
/
Endotelio Vascular
/
Soluciones Preservantes de Órganos
Límite:
Aged
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Biol Regul Homeost Agents
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Italia