Effect of intense, ultrashort laser pulses on DNA plasmids in their native state: strand breakages induced by in situ electrons and radicals.
Phys Rev Lett
; 106(11): 118101, 2011 Mar 18.
Article
en En
| MEDLINE
| ID: mdl-21469899
ABSTRACT
Single strand breaks are induced in DNA plasmids, pBR322 and pUC19, in aqueous media exposed to strong fields generated using ultrashort laser pulses (820 nm wavelength, 45 fs pulse duration, 1 kHz repetition rate) at intensities of 1-12 TW cm(-2). The strong fields generate, in situ, electrons and radicals that induce transformation of supercoiled DNA into relaxed DNA, the extent of which is quantified. Introduction of electron and radical scavengers inhibits DNA damage; results indicate that OH radicals are the primary (but not sole) cause of DNA damage.
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Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Radiólisis de Impulso
/
ADN
/
Radical Hidroxilo
/
Roturas del ADN de Doble Cadena
Idioma:
En
Revista:
Phys Rev Lett
Año:
2011
Tipo del documento:
Article
País de afiliación:
India