The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes.
Mol Cell Biochem
; 355(1-2): 217-22, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21553260
ABSTRACT
SAP155 is an essential component of the spliceosome and its phosphorylation is required for splicing catalysis, but little is known concerning its function and regulation during spermatogenesis in postnatal murine testes. We report that inhibition of dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) IA strongly suppressed the mitogen-stimulated SAP155 phosphorylation and constitutive splicing of IκB pre-mRNA as well as the proliferation of spermatogonial and Sertoli cells in cultures of the 6-day post partum testes and a spermatogonial cell line, but not in a Sertoli cell line. Our findings suggest that the active spliceosome, containing SAP155 phosphorylated by DYRKIA, performs pre-mRNA splicing in spermatogonia during testicular development.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
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Células de Sertoli
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Espermatogonias
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Testículo
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Proteínas Tirosina Quinasas
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Precursores del ARN
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Empalme del ARN
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Empalmosomas
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Ribonucleoproteína Nuclear Pequeña U2
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Proteínas Serina-Treonina Quinasas
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Japón