[Neuregulin promotes spermatogonia proliferation in mice].
Zhonghua Nan Ke Xue
; 17(5): 406-9, 2011 May.
Article
en Zh
| MEDLINE
| ID: mdl-21837948
ABSTRACT
OBJECTIVE:
To explore the role of neuregulin (neural regulation of protein, NRG) in the process of mouse spermatogonia proliferation.METHODS:
Mouse testis fragments were cultured in the medium DMEM containing purified NRG1beta or NRG3 at the concentration of 50, 100 and 200 ng/ml, respectively, followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.RESULTS:
Compared with the control group, neuregulin significantly promoted the proliferation of spermatogonia (P < 0.05). The proliferation rates of spermatogonia cultured in the medium with 50, 100 and 200 ng/ml of NRG13 were 1.69, 1.55 and 1.86 times, and those with 50, 100 and 200 ng/ml of NRG3 were 1.35, 1.54 and 2.11 times that of the control.CONCLUSION:
NRG1beta and NRG3 can promote the proliferation of mouse spermatogonia, and NRG is expected to be applied in the treatment of male infertility.
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Base de datos:
MEDLINE
Asunto principal:
Espermatogonias
/
Neurregulina-1
/
Péptidos y Proteínas de Señalización Intracelular
/
Proliferación Celular
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2011
Tipo del documento:
Article
País de afiliación:
China