MCF-7 cells as a three-dimensional model for the study of human breast cancer.
Tissue Eng Part C Methods
; 17(11): 1097-107, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-21882900
ABSTRACT
The increasing use of three-dimensional (3D) cell culture is because it reproduces in vitro results similar to in vivo results. Multicellular tumor spheroids generated in vitro exhibit important characteristics of avascular tumors, mainly with respect to tumor physiology and microenvironment. The interaction among cells in a tridimensional culture environment enhances cell differentiation and leads to luminal formation in some breast-derived cell cultures. The present work describes a method that permits luminal formation in breast adenocarcinoma cell (MCF-7)-derived spheroids in a 3D environment. In the proposed model, several relevant parameters, such as cell survival, apoptosis, autophagy, and E-cadherin expression, were analyzed to understand the organization of MCF-7 cells during different culture phases, including luminal and bud formation.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Tissue Eng Part C Methods
Asunto de la revista:
BIOTECNOLOGIA
/
HISTOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil