Role of angiotensin and endothelin in testicular ischemia reperfusion injury.
Int J Urol
; 19(3): 257-63, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-22168394
ABSTRACT
OBJECTIVES:
To determine whether angiotensin and endothelin have any role in testicular ischemia reperfusion injury by investigating the effects of the angiotensin converting enzyme inhibitor enalapril, selective non-peptide angiotensin-II type I blocker losartan and dual endothelin receptor blocker bosentan.METHODS:
Rats were anesthetized with thiopental sodium (50 mg/kg i.p.) before the operation. The left testicular artery and vein of rats were occluded for 1 h; before the bilateral orchiectomy, the organ was allowed to reperfuse for 3 h or 24 h. Enalapril (20 mg/kg i.p.), losartan (30 mg/kg i.p.), bosentan (10 mg/kg i.p.) or vehicle (saline) were given 30 min before reperfusion. Malondialdehyde level was measured in testicular tissue after 3 h of reperfusion. Histological examination was carried out after 24 h of reperfusion.RESULTS:
Ischemia reperfusion caused a significant increase in malondialdehyde level of ipsilateral testis, and histopathological injury in both ipsilateral and contralateral testes. Enalapril, losartan and bosentan treatments prevented the ischemia reperfusion-induced augmentation in malondialdehyde levels. Only bosentan treatment ameloriated ischemia reperfusion-induced histopathological alterations.CONCLUSIONS:
Endothelin might play a more important role in pathogenesis of testicular ischemia reperfusion injury when compared with angiotensin.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Testículo
/
Angiotensinas
/
Daño por Reperfusión
/
Endotelinas
/
Malondialdehído
Límite:
Animals
Idioma:
En
Revista:
Int J Urol
Asunto de la revista:
UROLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Turquía