[Roles of periostin in proliferation and migration of vascular smooth muscle cells and the effect of atorvastatin on them].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
; 37(7): 689-94, 2012 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-22886209
ABSTRACT
OBJECTIVE:
To investigate the expression of periostin in in vitro cultured vascular smooth muscle cells (VSMCs) induced by TGF-ß1 and the relationship between periostin expression and the migration and proliferation of the VSMCs. Further, to investigate the effects of atorvastatin on the above-mentioned processes and the molecular mechanisms of atorvastatin inhibition of TGF-ß1- induced periostin production.METHODS:
Rat aorta smooth muscle cells were cultivated by the method of tissue explants adherence. Cells of generation 3 to 6 were used as the experimental system. Primary cultured rat vascular smooth muscle cells were treated by TGF-ß1 and different concentrations of atorvastatin,Y-2763 (Rho kinase inhibitor), or atorvastatin plus MVA for 24 hours. The expression of periostin was measured by RT-PCR and Western blot. A Boyden chamber assay was used to measure cell migration, and an MTT test was used to measure cell proliferation.RESULTS:
Periostin expression in rat VSMCs stimulated by TGF-ß1 increased significantly (4.158 ± 0.515 vs 0.385 ± 0.031), VSMC migration(25 ± 4 vs 8 ± 2) and proliferation (0.85 ± 0.06 vs 0.32 ± 0.03) also increased significantly. Atorvastatin significantly inhibited TGF-ß1-induced periostin production in rat VSMCs, as well as VSMC migration and proliferation, in a dose-dependent manner. Rho kinase inhibitor Y-27632 significantly inhibited TGF-ß1-induced periostin production in rat VSMCs (2.082 ± 0.245). The inhibitory effect of atorvastatin on periostin upregulation induced by TGF-ß1 was reversed by mevalonate (3.838 ± 0.326).CONCLUSION:
Periostin can promote rat VSMC migration and proliferation. Atorvastatin inhibition of periostin expression induced by TGF-ß1 in VSMCs may be exerted by inhibition of the production of MVA and other isoprene compounds and by blocking the Rho/Rho kinase signaling pathway.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Pirroles
/
Moléculas de Adhesión Celular
/
Miocitos del Músculo Liso
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Ácidos Heptanoicos
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Músculo Liso Vascular
Límite:
Animals
Idioma:
Zh
Revista:
Zhong Nan Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China