[Investigation of functional groups of Cryptococcus albidus alpha-L-rhamnosidase].
Mikrobiol Z
; 74(4): 19-28, 2012.
Article
en Uk
| MEDLINE
| ID: mdl-23088096
ABSTRACT
The effect of cations, anions and specific chemical reagents 1-[3-(dimethylamino)propyl]-3-ethylcarbodimide methiodide, EDTA, o-phenantroline, dithiotreitol, L-cysteine, beta-mercaptoethanol, p-chlormercurybenzoate (p-ChMB), N-ethylmaleimide on the alpha-L-rhamnosidase activity of Cryptococcus albidus has been investigated. The essential role of Ag+ which inhibits the alpha-L-rhamnosidase activity by 72.5% was shown. Rhamnose at 1-5 mM protect the enzyme from the negative effect of Ag(+). It was expected that carboxyl group of C-terminal aminoacid and imidazole group of histidine would participate in the catalytic action of alpha-L-rhamnosidase on the basis of inhibition and kinetic analysis.
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Base de datos:
MEDLINE
Asunto principal:
Ramnosa
/
Plata
/
Proteínas Fúngicas
/
Cryptococcus
/
Glicósido Hidrolasas
Idioma:
Uk
Revista:
Mikrobiol Z
Asunto de la revista:
MICROBIOLOGIA
Año:
2012
Tipo del documento:
Article