Inhibition of leukemic U937 cell growth by induction of apoptosis, cell cycle arrest and suppression of VEGF, MMP-2 and MMP-9 activities by cytotoxin protein NN-32 purified from Indian spectacled cobra (Naja naja) venom.
Toxicon
; 65: 1-4, 2013 Apr.
Article
en En
| MEDLINE
| ID: mdl-23337397
ABSTRACT
A cytotoxin NN-32 (6.7 kDa) from Indian cobra (Naja naja) venom inhibited human leukemic U937 cell growth as observed by Trypan blue dye exclusion method and cytotoxicity was confirmed by MTT assay. NN-32 induced apoptosis of U937 cell and cell cycle arrest of sub-G1 phase were revealed by FACS analysis. Increased Bax/Bcl-2 ratio, increased caspase 3 and 9 activities, cleaved PARP, decreased VEGF, MMP-2 and MMP-9 activities were observed after NN-32 treatment of U937 cell. Antileukemic activity of NN-32 on U937 cell may be due to activation of apoptosis, arresting cell cycle and antiangiogenesis activities.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proliferación Celular
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Proteínas Cardiotóxicas de Elápidos
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Venenos Elapídicos
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Citostáticos
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Antineoplásicos
Límite:
Animals
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Humans
Idioma:
En
Revista:
Toxicon
Año:
2013
Tipo del documento:
Article
País de afiliación:
India