Fast and quantitative analysis of branched-chain amino acids in biological samples using a pillar array column.
Anal Bioanal Chem
; 405(25): 7993-9, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-23708693
ABSTRACT
In this study, a fast and quantitative determination method for branched-chain amino acids (BCAAs), namely leucine, isoleucine, and valine, was developed using a pillar array column. A pillar array column with low-dispersion turns was fabricated on a 20 × 20-mm(2) microchip using multistep ultraviolet photolithography and deep reactive ion etching. The BCAAs were fluorescently labeled with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), followed by reversed-phase separation on the pillar array column. The NBD derivatives of the three BCAAs and an internal standard (6-aminocaproic acid) were separated in 100 s. The calibration curves for the NBD-BCAAs had good linearity in the range of 0.4-20 µM, using an internal standard. The intra- and interday precisions were found to be in the ranges of 1.42-3.80 and 2.74-6.97%, respectively. The accuracies for the NBD-BCAA were from 90.2 to 99.1%. The method was used for the analysis of sports drink and human plasma samples. The concentrations of BCAAs determined by the developed method showed good agreements with those determined using a conventional high-performance liquid chromatography system. As BCAAs are important biomarkers of some diseases, these results showed that the developed method could be a potential diagnostic tool in clinical research.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Dispositivos Laboratorio en un Chip
/
Aminoácidos de Cadena Ramificada
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón