[Prokaryotic soluble expression of protein D of Haemophilus influenzae type b].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 27(2): 89-91, 2013 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-24044208
ABSTRACT
OBJECTIVE:
To express the recombinant D protein in prokaryotic expression system solubly and make preparation for producing D-carrier conjugate vaccine next step.METHODS:
The hpd gene fragment removed of signal peptide from genomic DNA of Hib CMCC was inserted into pET43. 1a. The recombinant plasmid was transformed to competent E. coli BL21 (DE3) for expression under induction of IPTG. The expressed recombination protein was precipitated with ammonium sulfate, purified by DEAE anion exchange column chromatography and identified for reactogenicity by Western Blot.RESULTS:
The expressed recombination protein, in a soluble form, constained about 50% of total somatic protein and showed specific reaction with the HIB antisera after preliminary purification.CONCLUSION:
The D protein recombined expression plasmid was constructed successfully and expressed D protein in prokaryotic cells in a solube form.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Inmunoglobulina D
/
Proteínas Portadoras
/
Haemophilus influenzae tipo b
/
Lipoproteínas
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Asunto de la revista:
VIROLOGIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China