Possible participation of acidic pH in bone resorption in middle ear cholesteatoma.
Laryngoscope
; 124(1): 245-50, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24122656
OBJECTIVES/HYPOTHESIS: The etiopathology of bone resorption in cholesteatoma is unclear. We studied pH in middle ear cholesteatoma tissue and the permeability of the cholesteatoma epithelium in an attempt to elucidate the mechanism of bone resorption in this disease. STUDY DESIGN: Laboratorial study. METHODS: Cholesteatoma tissue was collected from patients with primary acquired middle ear cholesteatoma. The pH of the keratin debris of cholesteatoma was measured using a pH meter. The cholesteatoma epithelium was examined under a confocal laser scanning microscope, and under a transmission electron microscope. Expression of filaggrin in the cholesteatoma tissue was explored by fluorescence immunohistochemistry and by quantitative reverse transcription-polymerase chain reaction. RESULTS: The pH of the keratin debris of cholesteatoma was acidic. The pH of the basal layer of the cholesteatoma epithelium was significantly lower than that of the antrum mucosa. Transmission electron microscope showed distinct penetration of lanthanum in the intercellular space of the basal, spinous, and granular layers of the cholesteatoma epithelium, but only a small amount of lanthanum in the granular layer in the normal skin. The expression of filaggrin mRNA was significantly lower in the cholesteatoma tissue than in the normal skin. CONCLUSIONS: These results indicate that acid leakage through the cholesteatoma epithelium probably participates in the resorption of the underlying bone structure. The increased permeability of the cholesteatoma epithelium may be explained by a decrease in filaggrin expression.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Resorción Ósea
/
Colesteatoma del Oído Medio
Límite:
Humans
Idioma:
En
Revista:
Laryngoscope
Asunto de la revista:
OTORRINOLARINGOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Vietnam