Phenotypical and molecular responses of Arabidopsis thaliana roots as a result of inoculation with the auxin-producing bacterium Azospirillum brasilense.
New Phytol
; 201(3): 850-861, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24219779
ABSTRACT
The auxin-producing bacterium Azospirillum brasilense Sp245 can promote the growth of several plant species. The model plant Arabidopsis thaliana was chosen as host plant to gain an insight into the molecular mechanisms that govern this interaction. The determination of differential gene expression in Arabidopsis roots after inoculation with either A. brasilense wild-type or an auxin biosynthesis mutant was achieved by microarray analysis. Arabidopsis thaliana inoculation with A. brasilense wild-type increases the number of lateral roots and root hairs, and elevates the internal auxin concentration in the plant. The A. thaliana root transcriptome undergoes extensive changes on A. brasilense inoculation, and the effects are more pronounced at later time points. The wild-type bacterial strain induces changes in hormone- and defense-related genes, as well as in plant cell wall-related genes. The A. brasilense mutant, however, does not elicit these transcriptional changes to the same extent. There are qualitative and quantitative differences between A. thaliana responses to the wild-type A. brasilense strain and the auxin biosynthesis mutant strain, based on both phenotypic and transcriptomic data. This illustrates the major role played by auxin in the Azospirillum-Arabidopsis interaction, and possibly also in other bacterium-plant interactions.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Azospirillum brasilense
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Arabidopsis
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Raíces de Plantas
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Ácidos Indolacéticos
Tipo de estudio:
Qualitative_research
País/Región como asunto:
America do sul
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Brasil
Idioma:
En
Revista:
New Phytol
Asunto de la revista:
BOTANICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Bélgica