Expression of transforming growth factor ß and fibroblast growth factor 2 in the lens epithelium of Morioka cataract mice.
Congenit Anom (Kyoto)
; 54(2): 104-9, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24279395
ABSTRACT
In the Morioka cataract (MCT) mice, lens opacity appears at 6 to 8 weeks of age, and swollen lens fiber is electron-microscopically observed at 3 weeks after birth. The present study was designed to characterize the expression of transforming growth factor ß (TGFß) and fibroblast growth factor 2 (FGF2) in the lens epithelium of the MCT mice. Immunohistochemical analysis showed that the expression of TGFß in the lens epithelium of the MCT mice was stronger than that of the wild-type ddY mice at 2 and 4 weeks after birth. The expression of TGFß receptors (TGFßRI and TGFßRII) and FGF2 in the lens epithelium of the MCT mice was stronger than that of the wild-type ddY mice at 4 weeks and weaker than that of the wild-type ddY mice at 15 weeks after birth. Using real time polymerase chain reaction (PCR), quantitative RT-PCR analysis showed that expression of TGFß1 and TGFß2 mRNA in the lens of 2-week-old MCT mice was significantly higher compared to age-matched wild-type ddY mice. These findings indicate that the lens epithelium of MCT mice has increased expression of TGFß before cataract affection and that changes in the expression of FGF2 as well as TGFß may contribute to the progression of the cataract in the mice.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Catarata
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Factor 2 de Crecimiento de Fibroblastos
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Proteínas Serina-Treonina Quinasas
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Receptores de Factores de Crecimiento Transformadores beta
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Factor de Crecimiento Transformador beta1
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Factor de Crecimiento Transformador beta2
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Congenit Anom (Kyoto)
Asunto de la revista:
TERATOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón