Alterations in the chondroitin sulfate chain in human osteoarthritic cartilage of the knee.
Osteoarthritis Cartilage
; 22(2): 250-8, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24280246
ABSTRACT
OBJECTIVE:
To determine whether the structure of chondroitin sulfate (CS) in cartilage is reflected by the degree of cartilage degeneration in patients with osteoarthritis (OA) of the knee and to determine how CS biosynthesis affects cartilage degeneration.DESIGN:
Two osteoarthritic cartilage samples were obtained from medial femoral condyle (MFC) and lateral femoral condyle (LFC) of 24 knees with end-stage OA. The samples were assigned to two groups as follows lesion and remote cartilage were adjacent to and remote from the osteoarthritic cartilage, respectively. Histological grade was determined according to the Mankin score. The CS concentration and chain length were determined using high-performance liquid chromatography (HPLC) and gel filtration chromatography, respectively. Expression of the gene encoding CS glycosyltransferase was evaluated using a real-time quantitative polymerase chain reaction (qPCR) assay. These results were compared between lesion and remote cartilage.RESULTS:
The Mankin score indicated that lesion cartilage was more degraded compared with remote cartilage. Although the CS levels varied among individuals, the mean CS concentration and chain length were significantly lower and shorter in lesion cartilage than in remote cartilage, respectively (concentration 12.04 vs 14.84 µg/mg wet weight, P = 0.021; chain length 5.36 vs 6.19 kDa, P = 0.026). Three genes encoding CS glycosyltransferases (CHPF, CSGALNACT1, CSGALNACT2) were expressed at lower levels in lesion cartilage.CONCLUSIONS:
In the osteoarthritic knee, the CS concentration and chain length were reduced closer to the more degraded cartilage with decreasing CS glycosyltransferase gene expression. Inhibition of CS glycosyltransferase gene expression may reduce CS chain length, which may contribute to OA progression.Palabras clave
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Cartílago Articular
/
Sulfatos de Condroitina
/
Condrocitos
/
Osteoartritis de la Rodilla
/
Articulación de la Rodilla
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
Límite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Osteoarthritis Cartilage
Asunto de la revista:
ORTOPEDIA
/
REUMATOLOGIA
Año:
2014
Tipo del documento:
Article