Infection of cultured human thymic epithelial cells by human immunodeficiency virus.
Clin Immunol Immunopathol
; 51(2): 185-95, 1989 May.
Article
en En
| MEDLINE
| ID: mdl-2467778
ABSTRACT
We have succeeded in growing the HTLV-IIIB strain of human immunodeficiency virus type 1 (HIV-1) in cultured human thymic epithelial (TE) cells. Expression of the HIV-1 proteins p17 and p24 was detected by immunofluorescence and reached a peak at 3 days after infection. Antigen capture and reverse transcriptase assays were used to detect HIV-1 in culture fluids, with positive results also being realized. The infection was cytolytic; cellular disarrangement, increased numbers of Hassall's corpuscles, and giant cells first appeared in monolayers of TE cells at 2 days after inoculation. By 4 days these changes were increased, and by 7 days, retraction and involution of TE cells were evident. The infection of TE cells by HIV-1 was blocked by preincubation with monoclonal OKT4A antibodies directed against CD4 target molecules.
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Base de datos:
MEDLINE
Asunto principal:
Timo
/
VIH
Límite:
Humans
Idioma:
En
Revista:
Clin Immunol Immunopathol
Año:
1989
Tipo del documento:
Article
País de afiliación:
Canadá