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Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray.
Moran, Sebastián; Vizoso, Miguel; Martinez-Cardús, Anna; Gomez, Antonio; Matías-Guiu, Xavier; Chiavenna, Sebastián M; Fernandez, Andrés G; Esteller, Manel.
Afiliación
  • Moran S; Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
  • Vizoso M; Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
  • Martinez-Cardús A; Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
  • Gomez A; Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain.
  • Matías-Guiu X; Department of Pathology and Molecular Genetics, and Research Laboratory; Arnau de Vilanova Universitary Hospital; University of Lleida IRBLLEIDA; Lleida, Catalonia, Spain.
  • Chiavenna SM; Ferrer Advanced Biotherapeutics; Barcelona, Catalonia, Spain.
  • Fernandez AG; Ferrer Advanced Biotherapeutics; Barcelona, Catalonia, Spain.
  • Esteller M; Cancer Epigenetics and Biology Program (PEBC); Bellvitge Biomedical Research Institute (IDIBELL); Barcelona, Catalonia, Spain; Department of Physiological Sciences II; School of Medicine; University of Barcelona; Barcelona, Catalonia, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA);
Epigenetics ; 9(6): 829-33, 2014 Jun.
Article en En | MEDLINE | ID: mdl-24732293
ABSTRACT
A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Metilación de ADN / Análisis de Secuencia por Matrices de Oligonucleótidos / Reparación del ADN Límite: Humans Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2014 Tipo del documento: Article País de afiliación: España
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Metilación de ADN / Análisis de Secuencia por Matrices de Oligonucleótidos / Reparación del ADN Límite: Humans Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2014 Tipo del documento: Article País de afiliación: España