Effect of hydration on plasmonic coupling of bioconjugated gold nanoparticles immobilized on a gold film probed by surface-enhanced Raman spectroscopy.
Langmuir
; 30(22): 6309-13, 2014 Jun 10.
Article
en En
| MEDLINE
| ID: mdl-24854627
ABSTRACT
Gold nanoparticle (AuNP)-Au film constructs were prepared using antibody-antigen interactions or a small organic cross-linker to systematically control the gap between the AuNP and Au film. Surface-enhanced Raman spectroscopy (SERS), scanning electron micrsocopy (SEM), and atomic force microscopy (AFM) were used to characterize each construct and elucidate structure-activity relationships. Interestingly, plasmonic coupling and SERS intensity were reversibly modulated with wetting/drying cycles for the protein immobilized AuNP, and this effect was attributed to changes in protein size with hydration state. This work provides insight into fundamental limitations of AuNP-enabled SERS bioassays and will facilitate rational design of novel biospecific ligands that maximize SERS sensitivity.
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1
Base de datos:
MEDLINE
Asunto principal:
Nanopartículas del Metal
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Oro
Idioma:
En
Revista:
Langmuir
Asunto de la revista:
QUIMICA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos