In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells.
Biosci Biotechnol Biochem
; 78(10): 1731-7, 2014.
Article
en En
| MEDLINE
| ID: mdl-25118018
ABSTRACT
Fresh loquat leaves have been used as folk health herb in Asian countries for long time, although the evidence supporting their functions is still minimal. This study aimed to clarify the chemopreventive effect of loquat tea extract (LTE) by investigating the inhibition on proliferation, and underlying mechanisms in human promyelocytic leukemia cells (HL-60). LTE inhibited proliferation of HL-60 in a dose-dependent manner. Molecular data showed that the isolated fraction of LTE induced apoptosis of HL-60 as characterized by DNA fragmentation; activation of caspase-3, -8, and -9; and inactivation of poly(ADP)ribose polymerase. Moreover, LTE fraction increased the ratio of pro-apoptotic Bcl-2-associated X protein (Bax)/anti-apoptotic myeloid cell leukemia 1 (Mcl-1) that caused mitochondrial membrane potential loss and cytochrome c released to cytosol. Thus, our data indicate that LTE might induce apoptosis in HL-60 cells through a mitochondrial dysfunction pathway. These findings enhance our understanding for chemopreventive function of loquat tea.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Bebidas
/
Extractos Vegetales
/
Leucemia
/
Depuradores de Radicales Libres
/
Apoptosis
/
Eriobotrya
/
Antineoplásicos
Límite:
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón