Your browser doesn't support javascript.
loading
Digital microfluidic processing of mammalian embryos for vitrification.
Pyne, Derek G; Liu, Jun; Abdelgawad, Mohamed; Sun, Yu.
Afiliación
  • Pyne DG; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.
  • Liu J; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.
  • Abdelgawad M; Department of Mechanical Engineering, Assiut University, Assiut, Egypt.
  • Sun Y; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.
PLoS One ; 9(9): e108128, 2014.
Article en En | MEDLINE | ID: mdl-25250666
Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Criopreservación / Microfluídica / Embrión de Mamíferos / Vitrificación Tipo de estudio: Guideline Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Criopreservación / Microfluídica / Embrión de Mamíferos / Vitrificación Tipo de estudio: Guideline Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2014 Tipo del documento: Article País de afiliación: Canadá