A novel multiplex assay amplifying 13 Y-STRs characterized by rapid and moderate mutation rate.
Forensic Sci Int Genet
; 15: 49-55, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25487077
ABSTRACT
As microsatellites located on Y chromosome mutate with different rates, they may be exploited in evolutionary studies, genealogical testing of a variety of populations and even, as proven recently, aid individual identification. Currently available commercial Y-STR kits encompass mostly low to moderately mutating loci, making them a perfect choice for the first two applications. Some attempts have been made so far to utilize Y-STRs to provide a discriminatory tool for forensic purposes. Although all 13 rapidly mutating Y-STRs were already multiplexed, no single assay based on single-copy markers allowing at least a portion of close male relatives to be differentiated from one another is available. To fill in the blanks, we constructed and validated an assay comprised of single-copy Y-STR markers only with a mutation rate ranging from 8×10(-3) to 1×10(-2). Performance of the resulting combination of nine RM Y-STRs and four moderately mutating ones was tested on 361 father-son pairs and 1326 males from 9 populations revealing an overall mutation rate of 1.607×10(-1) for the assay as a whole. Application of the proposed 13 Y-STR set to differentiation of haplotypes present among homogenous population of Buryats resulted in a threefold increase of discrimination as compared with 10 Y-STRs from the PowerPlex(®) Y.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Repeticiones de Microsatélite
/
Cromosomas Humanos Y
/
Mutación
Límite:
Humans
Idioma:
En
Revista:
Forensic Sci Int Genet
Asunto de la revista:
GENETICA
/
JURISPRUDENCIA
Año:
2015
Tipo del documento:
Article