Ovarian Hormones Regulate SP-D Expression in the Mouse Uterus During Estrous Cycle and Early Pregnancy.
Am J Reprod Immunol
; 74(1): 77-88, 2015 Jul.
Article
en En
| MEDLINE
| ID: mdl-25693975
ABSTRACT
PROBLEM:
Differential expression of SP-D in the cycling human and mouse endometrium suggests its regulation by ovarian hormones. METHOD OF STUDY SP-D expression in the mouse uterus was analyzed across the estrous cycle and during early pregnancy. Effect of exogenous ovarian hormones on the uterine expression of SP-D was analyzed.RESULTS:
SP-D expression varied across the estrous cycle and peaked in the estrous phase. SP-D transcript levels increased by fourfold in the uteri of estrogen-treated mice while co-administration of estrogen and progesterone enhanced SP-D levels by ninefold. However, treatment with progesterone alone significantly downregulated SP-D expression. Diethylstilbestrol enhanced SP-D transcript levels in the uteri of immature mice by 10-fold. During pregnancy, SP-D levels declined rapidly from 0.5 dpc to 6.5 dpc. In silico analysis predicted the presence of two potential ERE and 1 PRE in the mouse SP-D gene promoter region.CONCLUSION:
Estrogen positively regulates expression of SP-D in the mouse uterus. Progesterone, along with estrogen synergizes SP-D expression, however, when administered alone results in negative regulation.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Ovario
/
Progesterona
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Útero
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Proteína D Asociada a Surfactante Pulmonar
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Estrógenos
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2015
Tipo del documento:
Article
País de afiliación:
India