Open-frame system for single-molecule microscopy.
Rev Sci Instrum
; 86(3): 033701, 2015 Mar.
Article
en En
| MEDLINE
| ID: mdl-25832232
ABSTRACT
We present the design and construction of a versatile, open frame inverted microscope system for wide-field fluorescence and single molecule imaging. The microscope chassis and modular design allow for customization, expansion, and experimental flexibility. We present two components which are included with the microscope which extend its basic capabilities and together create a powerful microscopy system A Convex Lens-induced Confinement device provides the system with single-molecule imaging capabilities, and a two-color imaging system provides the option of imaging multiple molecular species simultaneously. The flexibility of the open-framed chassis combined with accessible single-molecule, multi-species imaging technology supports a wide range of new measurements in the health, nanotechnology, and materials science research sectors.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Imagen Molecular
/
Imagen Óptica
/
Microscopía
Idioma:
En
Revista:
Rev Sci Instrum
Año:
2015
Tipo del documento:
Article
País de afiliación:
Canadá