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Cost-efficient multiplex PCR for routine genotyping of up to nine classical HLA loci in a single analytical run of multiple samples by next generation sequencing.
Ozaki, Yuki; Suzuki, Shingo; Kashiwase, Koichi; Shigenari, Atsuko; Okudaira, Yuko; Ito, Sayaka; Masuya, Anri; Azuma, Fumihiro; Yabe, Toshio; Morishima, Satoko; Mitsunaga, Shigeki; Satake, Masahiro; Ota, Masao; Morishima, Yasuo; Kulski, Jerzy K; Saito, Katsuyuki; Inoko, Hidetoshi; Shiina, Takashi.
Afiliación
  • Ozaki Y; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. y-ozaki@tokai-u.jp.
  • Suzuki S; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. shingo@tokai-u.jp.
  • Kashiwase K; HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, Tokyo, 135-8639, Japan. k-kashiwase@ktks.bbc.jrc.or.jp.
  • Shigenari A; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. ashige@is.icc.u-tokai.ac.jp.
  • Okudaira Y; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. y-okuda@is.icc.u-tokai.ac.jp.
  • Ito S; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. s-ito@tokai-u.jp.
  • Masuya A; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. masuya@is.icc.u-tokai.ac.jp.
  • Azuma F; HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, Tokyo, 135-8639, Japan. f-azuma@ktks.bbc.jrc.or.jp.
  • Yabe T; HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, Tokyo, 135-8639, Japan. t-yabe@ktks.bbc.jrc.or.jp.
  • Morishima S; Department of Hematology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan. smorishi@fujita-hu.ac.jp.
  • Mitsunaga S; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. s-mitsu@is.icc.u-tokai.ac.jp.
  • Satake M; HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, Tokyo, 135-8639, Japan. m-satake@ktks.bbc.jrc.or.jp.
  • Ota M; Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, 390-8621, Japan. otamasao@shinshu-u.ac.jp.
  • Morishima Y; Division of Epidemiology and Prevention, Aichi Cancer Center Research Institute, Nagoya, Aichi, 464-8681, Japan. ymorisim@aichi-cc.jp.
  • Kulski JK; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. kulski@me.com.
  • Saito K; Centre for Forensic Science, The University of Western Australia, Nedlands, WA, 6008, Australia. kulski@me.com.
  • Inoko H; Research Department, One Lambda Inc, Part of Thermo Fisher Scientific, Kittridge Street, Canoga Park, CA, 91303-2801, USA. Yuki.Saito@thermofisher.com.
  • Shiina T; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, 259-1143, Japan. hinoko@is.icc.u-tokai.ac.jp.
BMC Genomics ; 16: 318, 2015 Apr 18.
Article en En | MEDLINE | ID: mdl-25895492
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Secuenciación de Nucleótidos de Alto Rendimiento / Técnicas de Genotipaje / Reacción en Cadena de la Polimerasa Multiplex / Antígenos HLA Tipo de estudio: Health_economic_evaluation Límite: Humans Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Secuenciación de Nucleótidos de Alto Rendimiento / Técnicas de Genotipaje / Reacción en Cadena de la Polimerasa Multiplex / Antígenos HLA Tipo de estudio: Health_economic_evaluation Límite: Humans Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Japón