The extended AT-hook is a novel RNA binding motif.
RNA Biol
; 12(8): 864-76, 2015.
Article
en En
| MEDLINE
| ID: mdl-26156556
ABSTRACT
The AT-hook has been defined as a DNA binding peptide motif that contains a glycine-arginine-proline (G-R-P) tripeptide core flanked by basic amino acids. Recent reports documented variations in the sequence of AT-hooks and revealed RNA binding activity of some canonical AT-hooks, suggesting a higher structural and functional variability of this protein domain than previously anticipated. Here we describe the discovery and characterization of the extended AT-hook peptide motif (eAT-hook), in which basic amino acids appear symmetrical mainly at a distance of 12-15 amino acids from the G-R-P core. We identified 80 human and 60 mouse eAT-hook proteins and biochemically characterized the eAT-hooks of Tip5/BAZ2A, PTOV1 and GPBP1. Microscale thermophoresis and electrophoretic mobility shift assays reveal the nucleic acid binding features of this peptide motif, and show that eAT-hooks bind RNA with one order of magnitude higher affinity than DNA. In addition, cellular localization studies suggest a role for the N-terminal eAT-hook of PTOV1 in nucleocytoplasmic shuttling. In summary, our findings classify the eAT-hook as a novel nucleic acid binding motif, which potentially mediates various RNA-dependent cellular processes.
Palabras clave
AKNAAT-hook-containing transcription factor; AT-hook; Brg1Brahma related gene 1; CaATL1Capsicum annuum AT-hook-Like gene 1; Dot1LDot1 [Disrupter of telomere silencing protein 1]-like; GPBP1; GPBP1GC-rich Promoter Binding Protein 1; HMGhigh mobility group; LEDGF/p75Lens epithelium-derived growth factor 75; MeCP2Methyl-CpG-binding protein 2; Med25Mediator complex subunit 25; PSR/JMJD6Phosphatidylserine receptorJmj-domain containing protein D6; PTOV1; PTOV1Prostate Tumor Overexpressed 1; RACK1receptor of protein C kinase 1; RNA-binding domain; TAF1TATA-binding protein-associated factor 1; TdIF1Terminal deoxynucleotidyltransferase-interacting factor 1; Tip5; Tip5TTF-I interacting protein 5; VL1Vascular protein family Vasculin-like 1; eAT-hookextended AT-hook; lncRNAlong non-coding RNA; microscale thermophoresis; protein-RNA interaction; rDNA EnrDNA enhancer; rDNA IGSrDNA intergenic spacer; snRNAsmall nuclear RNA
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ARN
/
Secuencias de Aminoácidos
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Proteínas de Unión al ADN
/
Motivos de Nucleótidos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
RNA Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania