Usefulness of in-house PCR methods for hepatitis B virus DNA detection.
J Virol Methods
; 223: 40-4, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26215428
ABSTRACT
The aim of the present study was to evaluate the performance of three in-house PCR techniques for HBV DNA detection and compare it with commercial quantitative methods to evaluate the usefulness of in-house methods for HBV diagnosis. Three panels of HBsAg reactive sera samples were evaluated (i) 50 samples were examined using three methods for in-house qualitative PCR and the Cobas Amplicor HBV Monitor Assay; (ii) 87 samples were assayed using in-house semi-nested PCR and the Cobas TaqMan HBV test; (iii) 11 serial samples obtained from 2 HBV-infected individuals were assayed using the Cobas Amplicor HBV test and semi-nested PCR. In panel I, HBV DNA was detected in 44 samples using the Cobas Amplicor HBV test, 42 samples using semi-nested PCR (90% concordance with Cobas Amplicor), 22 samples using PCR for the core gene (63.6% concordance) and 29 samples using single-round PCR for the pre-S/S gene (75% concordance). In panel II, HBV DNA was quantified in 78 of the 87 HBsAg reactive samples using Cobas TaqMan but 52 samples using semi-nested PCR (67.8% concordance). HBV DNA was detected in serial samples until the 17th and 26th week after first donation using in-house semi-nested PCR and the Cobas Amplicor HBV test, respectively. In-house semi-nested PCR presented adequate concordance with commercial methods as an alternative method for HBV molecular diagnosis in low-resource settings.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ADN Viral
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Virus de la Hepatitis B
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Reacción en Cadena de la Polimerasa
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Técnicas de Diagnóstico Molecular
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Hepatitis B
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Qualitative_research
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Virol Methods
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil