Residual γH2AX foci after ex vivo irradiation of patient samples with known tumour-type specific differences in radio-responsiveness.
Radiother Oncol
; 116(3): 480-5, 2015 Sep.
Article
en En
| MEDLINE
| ID: mdl-26297183
ABSTRACT
PURPOSE:
To apply our previously published residual ex vivo γH2AX foci method to patient-derived tumour specimens covering a spectrum of tumour-types with known differences in radiation response. In addition, the data were used to simulate different experimental scenarios to simplify the method. MATERIALS ANDMETHODS:
Evaluation of residual γH2AX foci in well-oxygenated tumour areas of ex vivo irradiated patient-derived tumour specimens with graded single doses was performed. Immediately after surgical resection, the samples were cultivated for 24h in culture medium prior to irradiation and fixed 24h post-irradiation for γH2AX foci evaluation. Specimens from a total of 25 patients (including 7 previously published) with 10 different tumour types were included.RESULTS:
Linear dose response of residual γH2AX foci was observed in all specimens with highly variable slopes among different tumour types ranging from 0.69 (95% CI 1.14-0.24) to 3.26 (95% CI 4.13-2.62) for chondrosarcomas (radioresistant) and classical seminomas (radiosensitive) respectively. Simulations suggest that omitting dose levels might simplify the assay without compromising robustness.CONCLUSION:
Here we confirm clinical feasibility of the assay. The slopes of the residual foci number are well in line with the expected differences in radio-responsiveness of different tumour types implying that intrinsic radiation sensitivity contributes to tumour radiation response. Thus, this assay has a promising potential for individualized radiation therapy and prospective validation is warranted.Palabras clave
Texto completo:
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Base de datos:
MEDLINE
Asunto principal:
Histonas
/
Neoplasias
Tipo de estudio:
Evaluation_studies
/
Observational_studies
Límite:
Female
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Humans
/
Male
Idioma:
En
Revista:
Radiother Oncol
Año:
2015
Tipo del documento:
Article