Predicting differential rifamycin resistance in clinical Mycobacterium tuberculosis isolates by specific rpoB mutations.
Int J Tuberc Lung Dis
; 19(10): 1222-6, 2015 Oct.
Article
en En
| MEDLINE
| ID: mdl-26459537
ABSTRACT
SETTING:
Rifampin (RMP) resistant Mycobacterium tuberculosis is usually assumed to be resistant to all rifamycins. Increasing evidence indicates, however, that some rpoB mutations, detectable by rapid molecular diagnostics, confer resistance to RMP but not to rifabutin (RBT), suggesting that RBT may be effective for the treatment of M. tuberculosis with these mutations.OBJECTIVE:
To determine if specific rpoB mutations reliably predict differential phenotypic resistance to RMP and RBT.DESIGN:
We selected 60 clinical M. tuberculosis isolates from a repository of multinational multidrug-resistant tuberculosis isolates and stratified them into two groups 1) those with rpoB mutations suspected to confer differential resistance to RMP and RBT, and 2) those expected to be cross-resistant to RMP and RBT. These assumptions were tested by comparing the phenotypic susceptibilities of RMP/RBT with those predicted by mutations in the rpoB gene.RESULTS:
Of 20 suspected RMP-resistant/RBT-susceptible isolates, 15 were RMP-resistant but RBT-susceptible, 3 were RMP- and RBT-susceptible, and 2 were cross-resistant to both RMP and RBT. In comparison, 40 of 40 suspected cross-resistant isolates were both RMP- and RBT-resistant.CONCLUSION:
Our data support the association between specific rpoB mutations and differential resistance of M. tuberculosis to RMP and RBT. Clinical studies are required to investigate the efficacy of RBT in the treatment of M. tuberculosis harboring these mutations.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Rifampin
/
Proteínas Bacterianas
/
ARN Polimerasas Dirigidas por ADN
/
Rifabutina
/
Antibióticos Antituberculosos
/
Mycobacterium tuberculosis
Tipo de estudio:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Int J Tuberc Lung Dis
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos