PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing.
Nat Commun
; 6: 10124, 2015 Dec 08.
Article
en En
| MEDLINE
| ID: mdl-26644285
ABSTRACT
In CRISPR-Cas9 genome editing, the underlying principles for selecting guide RNA (gRNA) sequences that would ensure for efficient target site modification remain poorly understood. Here we show that target sites harbouring multiple protospacer adjacent motifs (PAMs) are refractory to Cas9-mediated repair in situ. Thus we refine which substrates should be avoided in gRNA design, implicating PAM density as a novel sequence-specific feature that inhibits in vivo Cas9-driven DNA modification.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ARN Guía de Kinetoplastida
/
Edición de ARN
/
División del ADN
/
Motivos de Nucleótidos
/
Sistemas CRISPR-Cas
Límite:
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Canadá