Novel delivery of Chlorin e6 using anti-EGFR antibody tagged virosomes for fluorescence diagnosis of oral cancer in a hamster cheek pouch model.
Eur J Pharm Sci
; 83: 143-54, 2016 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-26709081
ABSTRACT
PURPOSE:
Overexpression of epidermal growth factor receptor (EGFR) is observed in oral squamous cell carcinoma (OSCC) and is associated with increased proliferation, metastasis and therapeutic resistance. We aim to develop a novel drug delivery system comprised of a photosensitizer Chlorin e6 (Ce6) that is encapsulated in a viral envelope and tagged with anti-EGFR antibody to target OSCC.METHODS:
Ce6 was encapsulated in both virosomes (Ce6-Vir) and virosomes tagged with anti-EGFR antibody (Ce6-Vir-EGFR'). In vitro studies were conducted to assess the cellular uptake and bioavailability of the photosensitizer in OSCC cells. Ce6 alone or in constructs was then administered in a hamster cheek pouch model and fluorescence imaging and spectroscopy was performed.RESULTS:
In vitro results showed that the uptake of Ce6-Vir-EGFR' was lower than that for Ce6-Vir and Ce6 possibly due to its large size. Nevertheless, in vivo results showed significant tumor specificity of Ce6-Vir-EGFR' compared to Ce6. The tumor to normal mucosa ratio showed that Ce6-Vir-EGFR' can successfully target OSCC lesions and therefore shows potential for use in fluorescence diagnosis of OSCC.CONCLUSIONS:
Both the virosome-Ce6 constructs were internalized by OSCC cells and successfully used for fluorescence imaging. Tagging with anti-EGFR antibody further improved the targeting ability toward OSCC.Palabras clave
7,12-Dimethylbenz[a]anthracene (PubChem CID: 6001); Anti-EGFR antibody; Chlorin e6; Chlorin e6 (PubChem CID: 5357355); Dimethyl sulfoxide (PubChem CID: 679); Dormicum (PubChem CID: 4192); Eosin (PubChem CID: 11048); Fluorescence diagnosis; Hamster cheek pouch model; Hematoxylin (PubChem CID: 320930); Hypnorm (PubChem CID: 171220); Oral squamous cell carcinoma; Virosomes
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Porfirinas
/
Neoplasias de la Boca
/
Fármacos Fotosensibilizantes
/
Receptores ErbB
/
Anticuerpos Monoclonales
/
Mucosa Bucal
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
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Humans
/
Male
Idioma:
En
Revista:
Eur J Pharm Sci
Asunto de la revista:
FARMACIA
/
FARMACOLOGIA
/
QUIMICA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Singapur