[Construction and characterization of Salmonella Pathogenicity Island 2 (SPI-2) deletion mutant of Salmonella Pullorum S06004].
Wei Sheng Wu Xue Bao
; 55(9): 1154-9, 2015 Sep 04.
Article
en Zh
| MEDLINE
| ID: mdl-26762028
ABSTRACT
OBJECTIVE:
To research the pathogenicity of Salmonella Pathogenicity Island 2 (SPI-2) deletion mutant of Salmonella Pullorum and preliminary explore the feasibility of developing safe attenuated Salmonella Pullorum candidate vaccine strain.METHODS:
The SPI-2 (-40 kb) deletion mutant of Salmonella Pullorum S06004 was constructed using the λ-red recombinant system. Then the biological characteristics such as growth rate, biochemical properties, genetic stability and virulence were evaluated between the deletion mutant strain S06004ΔSPI2 and its parent strain S06004.RESULTS:
S06004ΔSPI2 was successfully constructed. The growth rate and biochemical properties of S06004ΔSPI2 were consistent with those of its parent strain S06004. The mutant was stable with the deletion of SPI-2. Chicken lethal test showed that the LD50 of S06004ΔSPI2 was 252 times higher than the parent strain S06004.CONCLUSION:
The virulence of S06004ΔSPI2 was obviously attenuated. This study provided basic data for further study of the functions of SPI-2, and implied its potential to develop attenuated Salmonella vaccine.
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Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Salmonelosis Animal
/
Eliminación de Gen
/
Salmonella enterica
/
Islas Genómicas
Límite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Wu Xue Bao
Año:
2015
Tipo del documento:
Article