Rapid karyotyping of spontaneous abortions with trophoblastic villi.
Ann Acad Med Singap
; 18(1): 52-4, 1989 Jan.
Article
en En
| MEDLINE
| ID: mdl-2712519
ABSTRACT
24-hour short term culture of trophoblastic villi provided rapid and accurate karyotypes in spontaneous first trimester abortions. Good quality metaphases were obtained in 60.0% of villous cultures if these were set up within 8 hours of uterine evacuation, in contrast to the 10.3% success rate with intervals longer than 8 hours. 21% (4 of 19) of first trimester pregnancy losses were chromosomally abnormal, 3 of which were examples of autosomal trisomy; in 2 cases, the mothers were more than 37 years of age. The results of successful karyotyping reported here compare well with previous reports of long term culture of minced fetal material. Further, the risks of infected cultures, maternal contamination and pseudomosaicism are minimal with the short-term culture technique described here. Chorionic villi isolated from spontaneously aborted material are a simple and practicable source of fetal material for rapid and accurate cytogenic diagnosis in early spontaneous abortions.
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Base de datos:
MEDLINE
Asunto principal:
Muestra de la Vellosidad Coriónica
/
Aborto Espontáneo
/
Vellosidades Coriónicas
Límite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Ann Acad Med Singap
Año:
1989
Tipo del documento:
Article
País de afiliación:
Singapur