Engineering Escherichia coli to bind to cyanobacteria.
J Biosci Bioeng
; 123(3): 347-352, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-27773604
ABSTRACT
We engineered Escherichia coli cells to bind to cyanobacteria by heterologously producing and displaying lectins of the target cyanobacteria on their surface. To prove the efficacy of our approach, we tested this design on Microcystis aeruginosa with microvirin (Mvn), the lectin endogenously produced by this cyanobacterium. The coding sequence of Mvn was C-terminally fused to the ice nucleation protein NC (INPNC) gene and expressed in E. coli. Results showed that E. coli cells expressing the INPNCMvn fusion protein were able to bind to M. aeruginosa and the average number of E. coli cells bound to each cyanobacterial cell was enhanced 8-fold. Finally, a computational model was developed to simulate the binding reaction and help reconstruct the binding parameters. To our best knowledge, this is the first report on the binding of two organisms in liquid culture mediated by the surface display of lectins and it may serve as a novel approach to mediate microbial adhesion.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Adhesión Bacteriana
/
Ingeniería Genética
/
Microcystis
/
Escherichia coli
/
Bioingeniería
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China