Primorskyibacteraestuariivivens sp. nov., isolated from a tidal flat, and emended description of the genus Primorskyibacter.

ABSTRACT

A Gram-stain-negative, aerobic, non-spore-forming, motile and coccoid, ovoid or rod-shaped bacterial strain, designated OITF-36T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-36T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-36T clustered with the type strains of Primorskyibactersedentarius and Primorskyibacter insulae, showing 97.4-97.6 % sequence similarity. It exhibited sequence similarity values of less than 96.9 % to the type strains of the other recognized species. Strain OITF-36T contained Q-10 as the predominant ubiquinone and C18 1ω7c as the major fatty acid. The major polar lipids detected in strain OITF-36T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain OITF-36T was 61.8 mol%, and its mean DNA-DNA relatedness values with the type strains of P. sedentarius and P. insulae were 16-24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain OITF-36T is separate from P. sedentarius and P. insulae. On the basis of the data presented, strain OITF-36T is considered to represent a novel species of the genus Primorskyibacter, for which the name Primorskyibacteraestuariivivens sp. nov. is proposed. The type strain is OITF-36T (=KCTC 52455T=NBRC 112419T).