Tag-Free Semi-Synthesis of the Tau Protein.
Methods Mol Biol
; 1523: 215-235, 2017.
Article
en En
| MEDLINE
| ID: mdl-27975252
ABSTRACT
Expressed protein ligation (EPL) is a valuable tool to study site-specific functionalities on proteins such as posttranslational modifications. The purification of such ligation products from EPL mixtures can be cumbersome due to a small size difference between the expressed protein portion and the desired ligated protein. Therefore, affinity tags are often required, which remain on the protein after purification. Herein, we present an efficient protocol to install a photocleavable biotin building block on synthetic C-terminal tau[390-441] and describe its use for purification of full-length semi-synthetic tau[1-441].
Palabras clave
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Marcadores de Afinidad
/
Proteínas tau
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania