Acute upregulation of PGC-1α mRNA correlates with training-induced increases in SDH activity in human skeletal muscle.
Appl Physiol Nutr Metab
; 42(6): 656-666, 2017 Jun.
Article
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| MEDLINE
| ID: mdl-28177701
ABSTRACT
The purpose of the present study was to determine if acute responses in PGC-1α, VEGFA, SDHA, and GPD1-2 mRNA expression predict their associated chronic skeletal muscle molecular (SDH-GPD activity and substrate storage) and morphological (fibre-type composition and capillary density) adaptations following training. Skeletal muscle biopsies were collected from 14 recreationally active men (age 22.0 ± 2.4 years) before (PRE) and 3 h after (3HR) the completion of an acute bout of sprint interval training (SIT) (eight 20-s intervals at â¼170% peak oxygen uptake work rate separated by 10 s of recovery). Participants then completed 6 weeks of SIT 4 times per week with additional biopsies after 2 (MID) and 6 (POST) weeks of training. Acute increases in PGC-1α mRNA strongly predicted increases in SDH activity (a marker of oxidative capacity) from PRE and MID to POST (PRE-POST r = 0.81, r2 = 0.65, p < 0.01; MID-POST r = 0.79, r2 = 0.62, p < 0.01) and glycogen content from MID to POST (r = 0.60, r2 = 0.36, p < 0.05). No other significant relationships were found between acute responses in PGC-1α, VEGFA, SDHA, and GPD1-2 mRNA expression and chronic adaptations to training. These results suggest that acute upregulation of PGC-1α mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations. Additionally, acute mRNA responses in PGC-1α correlated with VEGFA, but not SDHA, suggesting a coordinated upregulation between PGC-1α and only some of its proposed targets in human skeletal muscle.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Succinato Deshidrogenasa
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Ejercicio Físico
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Músculo Esquelético
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Coactivador 1-alfa del Receptor Activado por Proliferadores de Peroxisomas gamma
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Appl Physiol Nutr Metab
Asunto de la revista:
CIENCIAS DA NUTRICAO
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FISIOLOGIA
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MEDICINA ESPORTIVA
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METABOLISMO
Año:
2017
Tipo del documento:
Article