Characterization of the selective alkylation site in hemoglobin A by dihydroartemisinin with tandem mass spectrometry.
Int J Biol Macromol
; 99: 358-364, 2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-28259625
ABSTRACT
The reaction between the antimalarial drug dihydroartemisinin (DHA) and hemoglobin A (HbA) was investigated in vitro. A fluorescein-tagged artemisinin analog reacted with HbA and fluorescent HbA-drug adducts could be visualized on SDS-PAGE to confirm stable covalent reaction adducts and necessity of the endoperoxide moiety and Fe(II). Mass spectrometric analyses revealed that DHA favourably alkylated protein part rather than heme and the modification site was identified to be at Tyr35 of the beta globin chain.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Hemoglobina A
/
Artemisininas
/
Antimaláricos
Límite:
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Tailandia