Construction of a Gene Knockdown System Based on Catalytically Inactive ("Dead") Cas9 (dCas9) in Staphylococcus aureus.

Zhao, Changlong; Shu, Xueqin; Sun, Baolin

Zhao, Changlong; Shu, Xueqin; Sun, Baolin.

Afiliación

Zhao C; CAS Key Laboratory of Innate Immunity and Chronic Disease and School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China.
Shu X; CAS Key Laboratory of Innate Immunity and Chronic Disease and School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China.
Sun B; CAS Key Laboratory of Innate Immunity and Chronic Disease and School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, China sunb@ustc.edu.cn.

Appl Environ Microbiol ; 83(12)2017 06 15.

Article en En | MEDLINE | ID: mdl-28411216

Asunto(s)

Técnicas de Silenciamiento del Gen/métodos; Staphylococcus aureus/genética; Proteínas Bacterianas/genética; Proteínas Bacterianas/metabolismo; Proteína 9 Asociada a CRISPR; Endonucleasas/genética; Endonucleasas/metabolismo; Técnicas de Silenciamiento del Gen/instrumentación; Silenciador del Gen; Operón; ARN Guía de Kinetoplastida/genética; ARN Guía de Kinetoplastida/metabolismo; Staphylococcus aureus/metabolismo

Palabras clave

CRISPRi; Staphylococcus aureus; dCas9; gene knockdown

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Técnicas de Silenciamiento del Gen Tipo de estudio: Evaluation_studies Idioma: En Revista: Appl Environ Microbiol Año: 2017 Tipo del documento: Article País de afiliación: China

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