Albumin conformational change and aggregation induced by nanostructured apatites.
Biointerphases
; 12(2): 02D403, 2017 05 01.
Article
en En
| MEDLINE
| ID: mdl-28460528
ABSTRACT
Biomaterials with nanostructured surfaces influence cellular response in a significantly different, and often beneficial, manner compared to materials with coarser features. Hydroxyapatite [HA, Ca10(PO4)6(OH)2] and strontium-apatite [Sr10(PO4)6(OH)2] microspheres that present nanotopographies similar to biological apatites were incubated in albumin solutions, at physiological conditions (40 mg ml-1; 37 °C), for up to 72 h. Electronic and vibrational circular dichroism spectroscopies revealed spectral signatures characteristic of stacked ß-sheet regions in higher ordered structures (e.g., fibrils). The presence of stacked ß-sheets was further evidenced by thioflavin T staining. The sequestration of interfacial Ca atoms by pyrophosphate ions (P2O74-), prior to albumin adsorption, prevented stacked ß-sheet formation on hydroxyapatite. These results suggest that the charge and/or spatial arrangement of Ca atoms direct stacked ß-sheet formation during bovine serum albumin adsorption. Stacked ß-sheet spectral features were also observed after incubating HA in fetal bovine serum, highlighting that this phenomena could direct cellular response to these biomaterials in vivo.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Albúmina Sérica Bovina
/
Durapatita
/
Nanoestructuras
Límite:
Animals
Idioma:
En
Revista:
Biointerphases
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2017
Tipo del documento:
Article