[The Effect of Ruxolitinib on the Expression of VEGF and HIF-1α in Leukemia HEL Cells].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 47(5): 669-673, 2016 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-28598077
ABSTRACT
OBJECTIVES:
To investigate the effect of Ruxolitinib on the expression of vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1 α (HIF-1α) in HEL cells.METHODS:
he HEL cells were treated with Ruxolitinib in different concentrations (1 nmol/L, 5 nmol/L, 10 nmol/L, 50 nmol/L, 100 nmol/L, 500 nmol/L). The growth inhibition of Ruxolitinib on HEL cells was detected by CCK-8 assay;the mRNA expression level ofJAK2 were measured by RT-PCR and the protein level of p-JAK2, VEGF, HIF-1α were observed by Western blot after treated with Ruxolitinib for 24,48,72 h. Chick chorioallantoic membrane (CAM) test was used to testify the effect of Ruxolitinib on angiogenesis.RESULTS:
Ruxolitinib with different concentrations could inhibit HEL cells proliferation. RT-PCR showed that the mRNA level ofJAK2 decreased in a concentration-dependent manner and Western blot demonstrated that the expression levels of p-JAK2, VEGF and HIF-1α were lower in Ruxolitinib treatment groups than those in control group (P<0.05) after HEL cells were treated with different concentrations of Ruxolitinib for 24,48,72 h. Ruxolitinib significantly suppressed blood vessels'formation in CAM.CONCLUSIONS:
Ruxolitinib can inhibit VEGF, HIF-1α expression and angiogenesis of HEL leukemia cells by inhibiting JAK2 pathway.Palabras clave
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Base de datos:
MEDLINE
Asunto principal:
Pirazoles
/
Leucemia
/
Factor A de Crecimiento Endotelial Vascular
/
Subunidad alfa del Factor 1 Inducible por Hipoxia
Límite:
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2016
Tipo del documento:
Article
País de afiliación:
China