Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid.
Methods Mol Biol
; 1643: 23-31, 2017.
Article
en En
| MEDLINE
| ID: mdl-28667527
ABSTRACT
Different protocols are required for the collection and isolation of antibodies from various body sites. For the sample collection factors to be considered include anatomic or physiological particularities. Secretory fluids such as saliva, gastrointestinal fluid, or breast milk may contain degrading enzymes that potentially affect the integrity of isolated antibodies. While the isolation of IgG from plasma is a common and often-described procedure, here we focus on methodological approaches to isolate antibodies immunoglobulin A (IgA) or IgM from plasma or secretory fluids. These protocols shall facilitate research on natural and induced antibodies.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Plasma
/
Saliva
/
Jugo Gástrico
/
Secreciones Intestinales
/
Leche Humana
/
Anticuerpos
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suiza