Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty.
Mol Cell Endocrinol
; 460: 104-122, 2018 01 15.
Article
en En
| MEDLINE
| ID: mdl-28711606
ABSTRACT
Although estrogens have been generally considered to play a critical role in ovarian differentiation in non-mammalian vertebrates, the specific functions of estrogens during ovarian differentiation remain unclear. We isolated two mutants with premature stops in the ovarian aromatase (cyp19a1) gene from an N-ethyl-N-nitrosourea-based gene-driven mutagenesis library of the medaka, Oryzias latipes. In XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles that failed to accumulate yolk. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues on the dorsal side of ovaries. In the newly formed testicular tissue, strong expression of gsdf was detected in sox9a2-positive somatic cells surrounding germline stem cells suggesting that gsdf plays an important role in testicular differentiation during estrogen-depleted female-to-male sex reversal. We conclude that endogenous estrogens synthesized after fertilization are not essential for early ovarian differentiation but are critical for the maintenance of adult ovaries.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Ovario
/
Maduración Sexual
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Oryzias
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Procesos de Determinación del Sexo
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Mutación con Pérdida de Función
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Endocrinol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón