TGF-ß1 is Involved in Vitamin D-Induced Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Regulating the ERK/JNK Pathway.
Cell Physiol Biochem
; 42(6): 2230-2241, 2017.
Article
en En
| MEDLINE
| ID: mdl-28817810
ABSTRACT
BACKGROUND/AIMS:
Osteoarthritis (OA) is characterized by degradation of cartilage, sole cell type of which is chondrocytes. Bone marrow-derived mesenchymal stem cells (BMSCs) possess multipotency and can be directionally differentiated into chondrocytes under stimulation. This study was aimed to explore the possible roles of vitamin D and transforming growth factor-ß1 (TGF-ß1) in the chondrogenic differentiation of BMSCs.METHODS:
BMSCs were isolated from femurs and tibias of rats and characterized by flow cytometry. After stimulation with vitamin D, BMSC proliferation and migration were measured by Cell Counting Kit-8 (CCK-8) and Transwell assays, respectively. Chondrogenic differentiation was estimated through expression levels of specific markers by qRT-PCR and Western blot analysis. After stable transfection, the effects of aberrantly expressed TGF-ß1 on vitamin D-induced alterations, including BMSC viability, migration and chondrogenic differentiation, were all evaluated utilizing CCK-8 assay, Transwell assay, qRT-PCR and Western blot analysis. Finally, the phosphorylation levels of key kinases in the extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) pathways were determined by Western blot analysis.RESULTS:
Vitamin D remarkably promoted BMSC viability, migration and chondrogenic differentiation. These alterations of BMSCs induced by vitamin D were reinforced by TGF-ß1 overexpression while were reversed by TGF-ß1 silencing. Additionally, the phosphorylation levels of ERK, JNK and c-Jun were enhanced by TGF-ß1 overexpression but were reduced by TGF-ß1 knockdown.CONCLUSION:
Vitamin D promoted BMSC proliferation, migration and chondrogenic differentiation. TGF-ß1 might be implicated in the vitamin D-induced alterations of BMSCs through regulating ERK/JNK pathway.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Vitamina D
/
Diferenciación Celular
/
Condrogénesis
/
Quinasas MAP Reguladas por Señal Extracelular
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Factor de Crecimiento Transformador beta1
/
Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
Cell Physiol Biochem
Asunto de la revista:
BIOQUIMICA
/
FARMACOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China