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High-throughput construction of multiple cas9 gene variants via assembly of high-depth tiled and sequence-verified oligonucleotides.
Cho, Namjin; Seo, Han Na; Ryu, Taehoon; Kwon, Euijin; Huh, Sunghoon; Noh, Jinsung; Yeom, Huiran; Hwang, Byungjin; Ha, Heejeong; Lee, Ji Hyun; Kwon, Sunghoon; Bang, Duhee.
Afiliación
  • Cho N; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
  • Seo HN; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
  • Ryu T; Celemics Inc., 371-17, Gasan-dong, Geumcheongu, Seoul 153-718, Republic of Korea.
  • Kwon E; Celemics Inc., 371-17, Gasan-dong, Geumcheongu, Seoul 153-718, Republic of Korea.
  • Huh S; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
  • Noh J; Department of Electrical and Computer Engineering, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
  • Yeom H; Department of Electrical and Computer Engineering, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
  • Hwang B; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
  • Ha H; Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
  • Lee JH; Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
  • Kwon S; Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul 02447, Republic of Korea.
  • Bang D; Department of Electrical and Computer Engineering, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Nucleic Acids Res ; 46(9): e55, 2018 05 18.
Article en En | MEDLINE | ID: mdl-29529247
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Oligonucleótidos / Proteína 9 Asociada a CRISPR / Genes Sintéticos Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Oligonucleótidos / Proteína 9 Asociada a CRISPR / Genes Sintéticos Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article