NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs.
Microbes Infect
; 20(5): 308-311, 2018 05.
Article
en En
| MEDLINE
| ID: mdl-29626632
ABSTRACT
Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analyzed. V2V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were identified in five PWID. For three of these patients, the reconstructed variants belonged to pure F1 subtype and CRF14_BG strains according to phylogenetic analysis. New recombinant forms between these parental strains were identified in two PWID samples. NGS data can provide, with the help of phylogenetic analysis, important insights about the intra-host sub-population structure.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Sobreinfección
/
Infecciones por VIH
/
VIH-1
/
Consumidores de Drogas
Límite:
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Microbes Infect
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Rumanía