Exosomeencapsulated microRNA23b as a minimally invasive liquid biomarker for the prediction of recurrence and prognosis of gastric cancer patients in each tumor stage.
Oncol Rep
; 40(1): 319-330, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29749537
ABSTRACT
Recently, exosomeencapsulated microRNAs (miRNAs) have been attracting attention as stable and minimally invasive biomarkers in cancer patients. The aim of the present study was to clarify the value of plasma exosomal microRNA23b (miR23b) as a diagnostic and prognostic biomarker in gastric cancer (GC) patients at each tumor stage. We first selected recurrence specific exosomal miRNA by miRNA microarray from 6 GC patients (stage I) with or without recurrence, and 3 healthy volunteers. In this analysis, miR23b demonstrated the most significant change. Subsequently, we validated the usefulness of miR23b as a biomarker using the plasma exosome samples collected from 232 GC patients and 20 healthy volunteers. miR23b levels were evaluated by Taqman microRNA assays. Exosomal miR23b levels of GC patients were significantly lower than those of the healthy controls. A significant association was revealed between the plasma exosomal miR23b levels and the expression of miR23b in primary tumor tissues. Concerning the pathological condition, miR23b demonstrated a significant association with tumor size, depth of invasion, liver metastasis and TNM stage. The overall survival (OS) rates of lowmiR23b patients were significantly worse than those of highmiR23b patients at stage I, II, III and IV. The diseasefree survival (DFS) rates of low exosomal miR23b patients were significantly worse than those of highmiR23b patients at stage I, II and III. Cox multivariate analysis indicated that exosomal miR23b was an independent prognostic factor for OS and DFS at each tumor stage. Our results revealed that exosomal miR23b has potential as minimally invasive predictive biomarker for the recurrence and prognosis of GC in patients at all stages.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Pronóstico
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Neoplasias Gástricas
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Biomarcadores de Tumor
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MicroARNs
Tipo de estudio:
Prognostic_studies
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Risk_factors_studies
Límite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2018
Tipo del documento:
Article
País de afiliación:
Japón