Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site.

Kokkonen, Piia; Bednar, David; Dockalova, Veronika; Prokop, Zbynek; Damborsky, Jiri

Kokkonen, Piia; Bednar, David; Dockalova, Veronika; Prokop, Zbynek; Damborsky, Jiri.

Afiliación

Kokkonen P; Loschmidt Laboratories, Department of Experimental Biology, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech R
Bednar D; Loschmidt Laboratories, Department of Experimental Biology, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech R
Dockalova V; Loschmidt Laboratories, Department of Experimental Biology, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech R
Prokop Z; Loschmidt Laboratories, Department of Experimental Biology, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech R
Damborsky J; Loschmidt Laboratories, Department of Experimental Biology, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Centre for Clinical Research, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech R

J Biol Chem ; 293(29): 11505-11512, 2018 07 20.

Article en En | MEDLINE | ID: mdl-29858243

Asunto(s)

Cumarinas/metabolismo; Hidrolasas/metabolismo; Xanthobacter/enzimología; Dominio Catalítico; Cumarinas/química; Cristalografía por Rayos X; Dicloruros de Etileno/metabolismo; Halogenación; Hidrolasas/química; Cinética; Metilación; Simulación del Acoplamiento Molecular; Simulación de Dinámica Molecular; Conformación Proteica; Especificidad por Sustrato; Xanthobacter/química; Xanthobacter/metabolismo

Palabras clave

active site; conformational change; dichloroethane degradation; enzyme catalysis; enzyme kinetics; enzyme mechanism; ethylene dichloride; haloalkane dehalogenase; molecular dynamics; molecular evolution; organic halogen; organohalogen; protein conformation

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Cumarinas / Xanthobacter / Hidrolasas Idioma: En Revista: J Biol Chem Año: 2018 Tipo del documento: Article

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Cumarinas / Xanthobacter / Hidrolasas Idioma: En Revista: J Biol Chem Año: 2018 Tipo del documento: Article