Hepatoprotective effect of lawsone on rifampicin-isoniazid induced hepatotoxicity in in vitro and in vivo models.
Environ Toxicol Pharmacol
; 61: 87-94, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29859372
ABSTRACT
The Drug-induced liver injury is one of the common unfavourable impacts, which seriously affects any drug therapy. This study documented the hepatoprotective efficacy of lawsone, the major bioactive naphthoquinone present in Lawsonia inermis L. (Lythraceae) using in vitro and in vivo models. Lawsone was isolated from the leaves of L. inermis and its structure was confirmed using spectroscopic data. In-vitro antioxidant effect of lawsone was evaluated using ABTS assay. Hepatoprotective effect of lawsone was determined with RIF-INH treated HepG2 cells and Wistar rats. Administration of RIF-INH reduced the viability of the HepG2 cells and the treatment with lawsone significantly restored the viability of the cells even at lower concentration (7.5 µM). The other parameters such as the leakage of transaminases and MDA levels were also significantly reduced by the treatment with lawsone. Oral administration of lawsone to the animals did not show any toxicity up to 2â¯g/kg b.w. concentration. Treatment with lawsone to the RIF-INH administered animals significantly lowered the serum transaminases levels. The ratio of albumin to globulin was improved and the level of bilirubin was lowered. This study indicated the hepatoprotective effect of lawsone; detailed investigations will give deeper understanding of the application of lawsone for hepatoprotection.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Naftoquinonas
/
Sustancias Protectoras
/
Enfermedad Hepática Inducida por Sustancias y Drogas
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Environ Toxicol Pharmacol
Año:
2018
Tipo del documento:
Article