A new dual-targeting real-time RT-PCR assay for hepatitis D virus RNA detection.
Diagn Microbiol Infect Dis
; 92(2): 112-117, 2018 Oct.
Article
en En
| MEDLINE
| ID: mdl-29941366
ABSTRACT
In this study, a real-time reverse transcription-polymerase chain reaction (real time RT-PCR) assay targeting 2 genetic segments was established to detect HDV RNA. Utilizing the World Health Organization International Standard for Hepatitis D Virus RNA, the lower limit of detection was 575 IU/mL, and the linearity of quantification ranged from 575,000 IU/mL to 575 IU/mL. 384 HBsAg-positive samples collected from China were tested by this method and HDV antibody detection. Eleven samples were positive for anti-HDV IgG which may persist after HDV resolution, 6 samples were HDV RNA positive, and 5 samples were positive for anti-HDV IgM. This assay showed more sensitivity than the detection of anti-HDV IgM. These data demonstrate that the real-time RT-PCR assay for HDV RNA could be implemented in the clinical detection of HDV infection in chronic HBV-infected patients in China.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Hepatitis D
/
Virus de la Hepatitis Delta
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Anticuerpos Antihepatitis
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Female
/
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2018
Tipo del documento:
Article