Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus.
Fish Shellfish Immunol
; 84: 756-767, 2019 Jan.
Article
en En
| MEDLINE
| ID: mdl-30368027
ABSTRACT
Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57⯱â¯4.37â¯nm) were moderately dispersed with a negative ζ potential of -10.3⯱â¯0.208â¯mV (pHâ¯=â¯7). AuNP were administered (single dose) at 0.2, 2, and 20⯵g/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24-48â¯h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2⯵g/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Vibrio parahaemolyticus
/
Adyuvantes Inmunológicos
/
Sustancias Protectoras
/
Penaeidae
/
Nanopartículas del Metal
/
Oro
Límite:
Animals
Idioma:
En
Revista:
Fish Shellfish Immunol
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
México